Theoretical Yield
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Peptide Synthesis Yield Calculator
Engineered for solid-phase peptide synthesis (SPPS), this tool instantly computes total available moles on resin and the theoretical yield of your target sequence.
Understanding SPPS Theoretical Yield
How to Use This Calculator
In Solid-Phase Peptide Synthesis (SPPS), the maximum amount of peptide you can theoretically generate is strictly limited by the number of active sites available on your polymer resin support (known as the resin loading value). This calculator computes exactly how much peptide you should expect to recover if every coupling step proceeds with 100% efficiency.
The Yield Formula
The standard equation for finding theoretical yield in solid-phase chemistry is:
Yield (mg) = Resin Loading (mmol/g) × Resin Weight (g) × Peptide Molecular Weight (mg/mmol)
Step-by-Step Explanation
- Determine the Scale: First, multiply the resin loading capacity (mmol/g) by the exact mass of the resin you weighed out (in grams). This gives you the total millimoles (mmol) of synthesis scale.
- Calculate the Mass: Multiply the scale (mmol) by the molecular weight of your fully synthesized peptide. Because molecular weight in g/mol is mathematically equivalent to mg/mmol, the resulting unit is exactly your expected theoretical yield in milligrams.
Important Considerations
- Cleavage Modifiers: Remember to calculate the molecular weight of the cleaved peptide (e.g., if it has an amidated C-terminus) rather than just summing the raw amino acid masses.
Frequently Asked Questions
What is "Resin Loading"?
Resin loading refers to the number of active sites on the polymer support per gram of resin, usually expressed as mmol/g. It determines the maximum theoretical scale of your synthesis.
Does this account for incomplete couplings?
No. This calculator computes the theoretical maximum yield assuming 100% coupling efficiency at every step. In practice, actual yields will be lower due to incomplete couplings and purification losses.
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